Analysis and study of growth hormone signal transduction pathway in elephant grass

Abstract

Elephant grass, also known as Napier grass, is important forage in Asia, Africa and the tropical and subtropical regions of the Americas, and is also a new type of energy plant. Elephant grass is widely used in agricultural production and industrial applications because of its well-developed root system, high light efficiency and high biomass. In order to reveal the genetic basis of its high biomass, we explored the auxin signal transduction pathway of elephant grass through bioinformatics methods. In this study, BlastP was used to identify 57 members of 6 gene families in the auxin signal transduction pathway of elephant grass cells. Chromosome localization analysis showed that 6 gene families were distributed on 14 chromosomes of elephant grass, and the B6 chromosome had the most genes. Protein molecular weight, isoelectric point, instability coefficient, fat solubility index and hydrophilic index of elephant grass were obtained by using TBtools Protein parameter calculation tool. Phylogenetic analysis showed that the homologous expression of biological functions of gene families in plants may be related to phylogenetic evolution. Selection pressure analysis showed that all gene families were not affected by positive selection, that is, no beneficial mutations occurred in the evolution process and were preserved by natural selection. Expression analysis showed that the expression levels of each gene were different in different tissues. Only a small amount of C3H family gene was detected in different tissues, and the other family genes were expressed in different developmental stages or different developmental parts of the roots and leaves, indicating that they participated in the whole development process of the roots and leaves of elephant grass, and played an important role in the regulation of growth and development of elephant grass. Protein interaction analysis showed that the genes were related to each other, and the two linked genes had similar biological functions, among which AUX/IAA family was the main core gene of elephant grass gene family protein network. The analysis of the signal transduction pathway of grass cytokinin in this study provides an important reference for further understanding of the growth and development mechanism of elephant grass, and also lays a foundation for future research on related genetic improvement and growth regulation.