Optimization of nutritional conditions for the synthesis of TG enzymes by fermentation by Streptomyces mobaraensis

Abstract

The purpose of this study was to optimize the nutritional conditions for the fermentation and synthesis of transglutaminase (TGase) by Streptomyces mobaraensis, so as to improve the yield and activity of TG enzyme. Streptomyces mobaraensis is a microorganism widely used in food processing and bioengineering with important potential for the production of transglutaminase (TG). In order to improve the efficiency of TG enzyme synthesis by Streptomyces mobaraensis, a series of single factor optimization experiments and response surface method were used to explore the effects of different nutritional conditions on the yield of TG enzyme. The experimental results show that Streptomyces mobaraensis produces the best results for TG enzyme at a growth temperature of 30°C and a pH value of 7.0. In terms of the carbon source, glycerol has been proven to have a significant effect on enhancing the yield of TG enzyme, and the highest TG enzyme yield is achieved when the concentration of glycerol is 15g/L. Additionally, the nitrogen source also plays an important role in the production of TG enzyme, and the addition of corn flour can significantly increase the yield of TG enzyme, with the optimal concentration being 20g/L. At the same time, the inoculation of 48-hour liquid seed, an initial pH value of 7.0, a cultivation temperature of 30°C, a shaking speed of 210 r/min, and a cultivation time of 48 hours were also determined to be the optimal fermentation conditions. The above optimization measures, by adjusting the culture medium and fermentation conditions, provide an important reference for the fermentation synthesis of TG enzyme by Streptomyces mobaraensis, which is expected to further improve the yield and activity of TG enzyme and promote its application in industrial production. The experimental results show that Streptomyces mobaraensis produces the best results for TG enzyme at a growth temperature of 30°C and a pH value of 7.0. In terms of the carbon source, glycerol has been proven to have a significant effect on enhancing the yield of TG enzyme, and the highest TG enzyme yield is achieved when the concentration of glycerol is 15g/L. Additionally, the nitrogen source also plays an important role in the production of TG enzyme, and the addition of corn flour can significantly increase the yield of TG enzyme, with the optimal concentration being 20g/L. At the same time, the inoculation of 48-hour liquid seed, an initial pH value of 7.0, a cultivation temperature of 30°C, a shaking speed of 210 r/min, and a cultivation time of 48 hours were also determined to be the optimal fermentation conditions. The above optimization measures, by adjusting the culture medium and fermentation conditions, provide an important reference for the fermentation synthesis of TG enzyme by Streptomyces mobaraensis, which is expected to further improve the yield and activity of TG enzyme and promote its application in industrial production..