Detection and exploration of the effect of Chaihu Shugan San on anxiety disorder using biotechnology protein immunoblotting method

Abstract

Li Tiantian. Detection and exploration of the effect of Chaihu Shugan San on anxiety disorder using biotechnology protein immunoblotting method. – Manuscript. Qualification thesis on the specialty 162 «Biotechnology and Bioengineering». – Kyiv National University of Technologies and Design, Kyiv, 2024. Anxiety is a common mental illness. It can seriously affect patients' quality of life. Traditional Chinese medicine believes that the main pathogenesis of anxiety is liver qi stagnation. Chaihu Shugan San is a commonly used Chinese herbal medicine, the main ingredients include bupleurum, peony, tuckahoe and so on. These herbs have a good effect of clearing heat and detoxifying and soothing liver and gallbladder. In this study, biotechnological methods were used to detect and validate bupleurum Shugan powder. In this study, normal C57BL/6J mice were selected and the gradient dose of Chaihu Shugan San was administered by intragastric administration. After the intragastric administration, behavioral evaluation methods were used: The Open Field Test (OFT) and Elevated Plus Maze (EPM) tests were conducted to determine the effect of the drug on anxiety-like behavior improvement in mice and to identify the optimal dose. Chronic restraint stress (CRS) was used to construct an anxiety model of mice, and Chaihu Shugan San was administered. Behavioral evaluation methods OFT and EPM were used to evaluate the efficacy of Chaihu Shugan San in the treatment of the anxiety model mice constructed by CRS. To explore whether changes in behavioral phenotype and the anti-anxiety effects of Chaihu Shugan San are related to Brain-derived neurotrophic factor (BDNF) in the hippocampus, In this study, the content of BDNF in the hippocampus of mice was detected by Western blot to reflect the pathological effect of Chaihu Shugan San on anxiety model mice. The experimental results showed that the anxiety symptoms of the treatment group were significantly alleviated, and the results of biochemical indicators supported the theory of traditional Chinese medicine. The effect of the treatment of anxiety was possible through clearing heat and detoxifying, soothing the liver and opening the gallbladder, and improving the stagnation of liver qi. However, the sample size of this study is small, and it is necessary to expand the sample in the later stage to further verify the conclusion. In general, this study adopts modern biotechnology methods to preliminarily detect and verify that Chaihu Shugan San may play a role in the treatment of anxiety through various ways.