Physiological differences of Populus wilsonii tissue cells based on gene expression

Abstract

Populus wilsonii is a tall tree belonging to the family of Salicaceae, mainly distributed in Shaanxi, ansu, Hubei, Sichuan, Yunnan, Tibet and other provinces in China. In this study, transcriptome analysis was erformed on buds, leaves, roots, xylem and phloem of Populus wilsonii samples to reveal functional differences of different tissues. The results showed that the number of clean reads in bud, leaf, phloem, root and xylem ranged from 4,515,553-36,645,427, and the genome coverage ranged from 77.77% to 95.46%. By analyzing the specific expression genes of different tissues, there were 447, 477, 167, 963 and 474 specific expression genes in the five sites, respectively. The most differentially expressed genes existed between middle tissues and other tissues, followed by root tissues and other tissues. These genes were mainly different in functions such as metabolism and protein transport. This study revealed the functional differences of various tissues from the aspect of gene expression, and provided a theoretical basis for further understanding of the physiological functions of the Populus wilsonii.