Bioinformatic analysis of Cenchrus purpureus genes for cytokinin signalling

Abstract

Elephant grass is an important pasture. It is a new type of energy plant, which is native to tropical and subtropical areas of Asia, Africa and America. It is a kind of high yield and forage grass, also can be used as feed for animals eat, widely used in agricultural production and industrial application. To reveal the development process of the genetic basis of cytokinin signal transduction, we through bioinformatics method to explore and analyze the elephant grass cytokinin signal transduction pathways of major gene family. This study using BlastP tools appraisal get elephant grass cytokinins three genes in signal transduction pathways of 20 members of the family; Protein characteristics analysis found that protein molecular weight between 8103-139430, isoelectric point between 5-10, unstable coefficient between 34-68, fat soluble index between 52-96 and hydrophilic index between 0.96 to 0.05; Gene structure analysis showed that the number of exons in different members of different families varied. Through the genetic analysis of chromosome positioning, found that elephant grass cytokinin signal transduction pathway 3 gene families, the three distribution on elephant grass 10 chromosomes gene families, in B6 gene on chromosome distribution; most Phylogenetic analysis through phylogenetic trees showed that the same gene families clustered together, which revealed the evolutionary relationship of organisms and better understood the similarities and differences between biological species. The expression levels of CRE1 family genes and B-ARR family genes were higher than those of AHP family genes in roots, while AHP family genes were mostly expressed in leaves. Protein interaction analysis revealed that the interaction between proteins was related to each other to understand the structure and function of the protein network. Among them, CRE1.8 was the main core gene of the elephant grass gene family protein network. This study provides an important reference for further understanding the growth and development mechanism of elephant grass, and lays a foundation for related genetic improvement and growth regulation research in the future. Elephant grass has been listed as a new energy plant in the 20th century, with important edible value, economic value and ecological value. By obtaining the cytokinin signal transduction pathway genes of purple elephant grass, we searched for the cytokinin signal transduction pathway genes of purple elephant grass, and analyzed the data of the studied genes by bioinformatics methods, so as to explore the important genes for the growth and development of purple elephant grass. By studying the elephant grass cytokinin signal transduction pathway, can have a thorough nderstanding of plant cytokinin signal transduction mechanism, revealing the molecular mechanism of plant growth and development. To explore the mechanism of plant adaptation to the environment, provide theoretical basis for the plant ecological adaptation and optimization, can provide theoretical support for plant breeding and production, to cultivate high yield, art, and provide a scientific basis for quality of crop varieties.